Dengue Virus NS1 Protein as a Diagnostic Marker: Commercially Available ELISA and Comparison to qRT-PCR and Serological Diagnostic Assays Currently Used by the State of Florida

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Dengue Virus NS1 Protein as a Diagnostic Marker: Commercially Available ELISA and Comparison to qRT-PCR and Serological Diagnostic Assays Currently Used by the State of Florida

BACKGROUND The proper management of patients infected with dengue virus requires early detection. Here, real-time molecular assays have proven useful but have limitations, whereas ELISAs that detect antibodies are still favored but results are obtained too late to be of clinical value. The production of DENV NS1 peaks early during infection and its detection can combine the advantages of both d...

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Serological Diagnostic Assays for Detection of Ns1 Antigen, IGM and IGG Antbodies to Dengue Virus

Complications in managing the dengue virus infections include the lack of rapid diagnostic procedures and at the same time the symptoms of dengue infection are often confused with those of other diseases. Two commercial rapid serological diagnostic kit methods (Dengue Day 1 test, J Mitra and Co. Pvt. Ltd., New Delhi, ImmunoComb II Dengue IgM/IgGBispot kit (Orgenics Pvt. Ltd., Israel)were evalua...

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Evaluation of Commercially Available Diagnostic Tests for the Detection of Dengue Virus NS1 Antigen and Anti-Dengue Virus IgM Antibody

Commercially available diagnostic test kits for detection of dengue virus (DENV) non-structural protein 1 (NS1) and anti-DENV IgM were evaluated for their sensitivity and specificity and other performance characteristics by a diagnostic laboratory network developed by World Health Organization (WHO), the UNICEF/UNDP/World Bank/WHO Special Programme for Research and Training in Tropical Diseases...

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Multi-Country Evaluation of the Sensitivity and Specificity of Two Commercially-Available NS1 ELISA Assays for Dengue Diagnosis

BACKGROUND Early diagnosis of dengue can assist patient triage and management and prevent unnecessary treatments and interventions. Commercially available assays that detect the dengue virus protein NS1 in the plasma/serum of patients offers the possibility of early and rapid diagnosis. METHODOLOGY/PRINCIPAL FINDINGS The sensitivity and specificity of the Pan-E Dengue Early ELISA and the Plat...

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ژورنال

عنوان ژورنال: Journal of Tropical Medicine

سال: 2017

ISSN: 1687-9686,1687-9694

DOI: 10.1155/2017/8072491